Labwork

Course part 1 - Laboratory (Monday)

• We selected and sub-cultured 20 isolates from the -80 freezer
  • 8 presumptive Salmonella spp. isolates
  • 6 presumptive E. coli isolates
  • 6 presumptive Klebsiella spp. isolates

Course part 2 - Laboratory (Wednesday)

• The Vitek machine was run on Tuesday (three times due to problems with the control)
• For most isolates the results were fine on the last run
  • No ID; no bacteria or not able to identify
  • We have not correlated the number and species ID from the VITEK to what was entered on the plates (would be nice to have the presumptive species to compare)

Picture of species identification results

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results1

Nine isolates were confirmed species ID for DNA extraction on Thursday; six E. coli and three Salmonella enterica:

E - Emmanuel, M - Maulid, A - Anna, C - Camilla

• • A2 - E
• • A4 - M
• • B3 - A
• • C1 - E
• • C2 - C
• • C4 - M
• • D1 - C
• • D2 - C
• • D4 - A

Sub-culturing of strains for DNA extraction was performed on Tuesday 19.11.2024